DNA

Part:BBa_K2100017:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR Taler21


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
    Illegal NotI site found at 421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 3382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 3509
    Illegal SpeI site found at 3452
    Illegal PstI site found at 117
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 2715
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is from the mammalian genome.

References